Biofilament Engineering

Various biological phenomena such as cell migration, cell differentiation, and tissue formation are governed by the structure of the protein network within or around the cell. We are interested in the engineering of the structure of the protein network by using physical and chemical stimuli. Our result will help to understand the behaviors of cells and tissues in physiological and pathological state and will be beneficial to develop biomimetic artificial tissue constructs for regenerative medicine and drug screening.

Featured images and movies of this topic

Engineering of actin protein network by using mechanical flow

Schematic of mechanical flow induced by surface acoustic wave (SAW) and fluorescent images of actin network aligned by the flow (Reference: Kang B, Jo S, Baek J, Nakamura F, Hwang W, Lee H. Role of Mechanical Flow for Actin Network Organization. Acta Biomaterialia, Vol. 90, P.217-24, 2019)

Analysis of polymerization and bundling of biofilament using photoluminescence

(Top) Schematic of the actin polymerization and bundling. (Bottom) Photoluminescence intensity of the actin during the polymerization and the bundling (Reference: Park B, Oh S, Jo S, Kang D, Lim J, Jung Y, Lee H, Jun SC. Determination of the molecular assembly of actin and actin-binding proteins using photoluminescence. Colloids and Surfaces B: Biointerfaces, Vol. 169, No. 1, P. 462-69, 2018)

Analysis of cellular dynamics on biomimetic substrate

SEM images of biomimetic collagen and single cell seeded on the biomimetic collagen

Movie of the tracking of cellular migration on biomimetic substrate